Bolton® Tubes contain a glucose stabiliser, which allows you to block the process of glycolysis for up to 24 hours at room temperature. Scope: research of glucose and lactate. Adding sodium fluoride and potassium oxalate to a tube helps prevent the breakdown of blood glucose (a process called glycolysis) and maintain its level in a blood sample.
Sodium fluoride and potassium oxalate act as anticoagulants, binding Ca2+ ions; in addition, sodium fluoride stabilises glucose levels. Glucose breaks down to pyruvate and lactate with the sequential implementation of various enzymatic reactions. Sodium fluoride inhibits some enzymatic reactions, including the conversion of phosphoglycerate to phosphoenolpyruvate, and prevents glycolysis.